WebJan 31, 2024 · Through this multifaceted approach, we show that Q513 and N414 are critical mediators of protein structural dynamics, linking the ultrafast (sub-ps) excitation of the FMN chromophore to the microsecond conformational changes that result in photoreceptor activation and biological function. Copyright WebThe flavin mononucleotide chromophore (FMN) is shown in the middle; the three residues subjected to point mutations (Ile532, Ala536, and Ile539), Gln513, and Trp491 are shown …
The FMN-binding domain of cytochrome P450BM-3: resolution ...
WebFeb 20, 2024 · Riboflavin-5′-monophosphate sodium salt hydrate (FMN) (Chemochroma), Lumichrome (Santa Cruz Biotechnology), tris (hydroxymethyl)aminomethane (Merck), … WebMay 31, 2013 · Specifically, the mutations improved quantum yield by relieving fluorescence quenching interactions between FMN and Phe37 and strengthening association between the protein and the FMN chromophore. In order to expand the scope and enhance the utility of FbFPs as practical fluorescent reporters, a comprehensive evaluation of the … sight and sound david show length
RCSB PDB - 4EES: Crystal structure of iLOV
WebMar 28, 2012 · Comparative structural analyses between iLOV and its progenitors reveal mutation-induced constraints in the environment of the flavin mononucleotide (FMN) chromophore; in iLOV, the methyl group … WebMar 28, 2012 · Comparative structural analyses between iLOV and its progenitors reveal mutation-induced constraints in the environment of the flavin mononucleotide (FMN) chromophore; in iLOV, the methyl group of Thr-394 "crowds" the FMN isoalloxazine ring, Leu-470 triggers side chain "flipping" of Leu-472, and the terminal FMN phosphate … WebMay 15, 2024 · Molecular modeling studies revealed the important amino acid residues that interact with the FMN chromophore. The chromophore was surrounded by metal … the pretender drum sheet music